The laboratory staff developed and implemented in the practice of experimental research a model of experimental colitis in different species of animals, a model of acute and chronic experimental pancreatitis, a model of chronic cholecystitis, a model of acute limited purulent staphylococcal endometritis in guinea pigs, a model of hepatic insufficiency, a model of chronic LPS-induced chronic neoplastic endotoxemia, a model of chronic neoplastic endogenous intoxication with induction of colon adenocarcinoma.

The results of experimental studies have allowed to develop new methods of detoxification and restorative therapy using modern sorption agents: plant sorption factors Carnitine chloride and Algigel, multifunctional carbon enterosorbents of IV generation GSHD and Carboline, as well as to investigate the effect of neoplastic intoxication on the cerebral cortex, to propose the determination of morphometric parameters of the spleen as an indirect marker of oncogenesis.

All new methods of experimental modeling of pathological processes and methods of their correction developed and implemented in the field of health care are protected by security documents of Ukraine.

Algorithm for selecting the timing and volume of surgery in patients with ulcerative duodenal hemorrhage based on immunomorphological assessment of the factors of protection of periulcerosis, ulcerative destruction, the likelihood of bleeding and the scale of surgical risk conditions of concomitant pathology was developed and implemented in the practice of surgical departments.

Endoscopic-immunomorphological monitoring of activity, dynamics and prognosis of duodenal ulcer complicated by bleeding, which is now widely used in clinical departments of surgery, is proposed.

In the period from 2010 to 2020, more than 350 dissertations were completed in Central Research Lab.

Of them:

§ 28 doctoral dissertations;

§ 350 candidate dissertations;

§ 7 research papers with state budget funding;

§ 9 economic contract research works.

During 2005-2021, the following fundamental and applied research was carried out in Central Research Lab with the financial support of the Ministry of Public Health of Ukraine:

§ Clinical, epidemiological and immunological substantiation of improvement of prophylactic and treatment technologies for influenza and other acute respiratory viral infections.

Supervisors:

Corresponding Member of the National Academy of Medical Sciences of Ukraine Professor L. Kovalchuk

Corresponding Member of the National Academy of Medical Sciences of Ukraine Professor M. Andreichyn

§ Morphofunctional bases of adaptation processes in cardiovascular and digestive systems in terms of arterial hypertension in big and small circles of blood circulation at various corrective influences.

Supervisor - Professor M. Hnatiuk

§ Structural and functional features of the small and large intestines in combined pathologies of the pancreatohepatobiliary zone.

Supervisor - Senior Researcher N. Lisnychuk

§ Pathogenic effect of polytrauma on the condition of internal organs and the development of ways of systemic correction.

Supervisor - Professor A. Hudyma

§ Correction of structural and functional changes of the small intestine under the combined pathologies of the pancreatohepatobiliary zone with the use of biospecific selective immunosorbents and magnetic laser radiation (experimental substantiation).

Supervisor - Senior Researcher N. Lisnychuk

§ Development of technology for the manufacture of crushed substrate of canned pig skin, its experimental and clinical studies for the treatment of immune-dependent pathology in adults and children.

Supervisor - Professor I. Klishch.

§ Development of differentiated methods of correction of hemodynamic and metabolic disorders in patients with arterial hypertension in combination with pathology of internal organs.

Supervisor - Professor M. Shved

§ Experimental study of the patterns of apoptosis in severe combined trauma.

Supervisor - Professor A. Gudyma.

§ Development of differentiated methods of correction of hemodynamic and metabolic disorders in patients with arterial hypertension in combination with pathology of internal organs.

Supervisor - Professor M. Shved

§ Investigation of the morphological condition of the kidney and large joints under the condition of combined pathologies of the pancreatohepatobiliary zone.

Supervisor - Senior Researcher N. Lisnychuk

§ Study of pathogenetic features of multiorgan failure development in the conditions of experimental cranioskeletal trauma and search for means of systemic correction by cell therapy.

Supervisor - Professor A. Gudyma.

§ Development of methods to increase the safety and effectiveness of surgical treatment of major surgical diseases of the abdominal cavity in conditions of polymorbidity.

Supervisor - Professor I. Dziubanovsky

§ Structural and metabolic changes of the cerebral cortex, kidneys and liver due to chronic neoplastic intoxication and components of cytostatic therapy and their sorption correction.

Supervisor - Senior Researcher N. Lisnychuk

§ Morphofunctional state of the spleen and factors of immunological reactivity in the conditions of experimental carcinogenesis.

Supervisor - Senior Researcher N. Lisnychuk

§ Study of age features of pathogenesis of cranioskeletal trauma and efficiency of its correction by cryopreserved fetal nerve cells.

Supervisor - Professor A. Gudyma.

§ Immunogenetics of malignant growth: the effect of BCL-2 gene expression on the state of antitumor immunity and under the corrective effect of nanoparticles.

Supervisor - Senior Researcher N. Lisnychuk

§ Immunogenetics of malignant growth: the effect of Bcl-2 gene expression on the state of antitumor immunity and under the corrective effect of nanoparticles.

§ Endothelial dysfunction, hypercoagulation and microvascular thrombosis in COVID-19: risk assessment, pathogenesis and diagnosis.

§ Investigation of the regenerative potential of cell therapy in acute respiratory distress syndrome.

§ Study of the effectiveness of the laminar pneumatic protection system of the mobile concept model of a pneumatic helmet against viral (coronavirus, COVID-19), bacterial, toxic agents.

§ Features of vitamin and mineral status in healthy children and children with socially significant diseases.

§ Multidisciplinary approach to pathogenetically reasonable and predictable choice of minimally invasive method of treatment of comorbid obesity and its effectiveness.

§ Development of specialized medical equipment and treatment/rehabilitation techniques for providing telemedicine (remote) care to patients with injuries and diseases of the musculoskeletal system.

§ Mono- and mixed tick-borne infections, improvement of medical and diagnostic technologies and biosafety measures.

§ Development of original combinations of antihypertensive drugs, their analysis and standardization.

§ A pilot study on neonatal screening of primary immunodeficiencies by the Trecs method for the detection of T-I and B-lymphopenia.

§ Introduction of elements of neuro-developmental care for premature babies and their follow-up.

§ Early diagnosis of cardiovascular and metabolic disorders in school-age children.

Areas of research provided in Central Research Lab:

study of patterns of pathological and adaptive-compensatory processes in experimental animals in osteoporosis, pathologies of the cardiovascular, respiratory and digestive systems, cancer, experimental substantiation of new methods of their prevention and correction, elucidation of the features of pro-and antiapoptotic proteins of the Bcl-2 family expression in the conditions of induced neoplastic lesions of the colon, the establishment of predictors of carcinogenic risks by immunological, immunohistochemical, hematological, biochemical and morphological diagnosis of comorbidity induced by chronic cancer, corrective effect of the composition of nanoparticles Ag/Fe/Au on the progression of malignancy in the colon.

Directions of further scientific research of Central Research Lab:

- Given the urgency of the safety of pharmacotherapy, it is advisable to conduct research to study the toxic effects of drugs on the function of internal organs and to search for effective means of correction and prevention;

- the prevalence and growth of cancer justifies the feasibility of studying structural and metabolic changes in the cerebral cortex, kidneys and liver due to chronic endogenous neoplastic intoxication and components of cytostatic therapy, as well as the development of pathogenetically adequate methods of prevention and treatment;

- study of the oxidative stress and nitric oxide system role in the pathogenesis of diseases of internal organs and search for effective means of their pharmacocorrection;

- elucidation of immunogenetic aspects of experimental neoplastic lesions development and features of their influence on functional activity of cellular and humoral factors of antitumor protection;

- study of the peculiarities of the course of immunoreactive processes (indicators of immunological resistance, changes in the cytokine profile and the processes of apoptosis and necrosis of CD3-lymphocytes) and assessment of the severity of metabolic disorders in experimental carcinogenesis;

- establishing the features of structural changes of lymphoid system organs developing in the conditions of experimental carcinogenesis at the cellular and subcellular levels and an estimation of degree of disturbance of histoarchitectonics of the spleen as the largest immunocompetent organ in the conditions of oncogenesis;

- evaluation of the effectiveness of the aqueous dispersion of silver, iron and gold combination nanoparticles on the expression of Bcl-2 family genes, indicators of immune reactivity (factors of humoral and cellular immunity, indicators of apoptosis and necrosis of CD3 lymphocytes), parameters of T-cytokine profile, IL-2, IL-4, IL-6, IL-10), metabolic disorders, manifestations of endogenous intoxication syndrome and oxidative stress factor - 8-isoprostaglandin F2α (8-isoprostane).

In 2010-2020, scientists of the CRL published:

Ø articles - 65 (including 15 articles in scientific journals indexed in Scopus and Web of Science Core Collection databases)

Ø thesis - 26 (including 18 abroad)

Ø patents of Ukraine - 12.

The innovation "Application of a complex of sorption and magnetolaser therapy in the rehabilitation treatment of patients with manifestations of reactive enteritis combined with pathologies of the pancreatohepatobiliary zone" developed by the research team of CRL is included in the Register of sectoral innovations № 32/33 for 2010 under № 50/32/10.

Published 10 innovations in the Health Care System:

§ Information letter № 112-2014 "Sorption correction of cytokine-mediated immunoreactive changes in the conditions of oncogenic endotoxemia" Yu. Soroka, I. Demkiv, N. Lisnychuk

§ Information letter № 113-2014 "Apoptosis-mediated changes in immune reactivity in conditions of adenocarcinomatosis of the colon induced by 1,2-dimethylhydrazine” Yu. Soroka, I. Demkiv, N. Lisnychuk

§ Information letter № 263-2014 "Ultrastructural changes of nephron components in the conditions of induced oncogenesis" Authors: N. Lisnychuk, K. Volkov, I. Demkiv, Z. Nebesna, Yu. Soroka

§ Information letter № 264-2014 "Structural changes of the liver in conditions of chronic neoplastic toxemia" K. Volkov, Yu. Soroka, I. Demkiv, N. Lisnychuk

§ Information letter № 141-2015 "Submicroscopic changes of the cerebral cortex in the conditions of chronic endotoxemia" Yu. Soroka, N. Lisnychuk, K. Volkov, Z. Nebesna, I. Demkiv

§ Information letter № 50-2015 "Ultrastructural changes of the alveoli of the respiratory lungs after experimental thermal trauma under conditions of correction" K. Volkov, Z. Nebesna, N. Lisnychuk, S. Kramar

§ Information letter № 50-2016 "Morphofunctional changes of the cerebral cortex in chronic neoplastic endotoxemia" N. Lisnychuk, K. Volkov, Z. Nebesna, I. Demkiv, Yu. Soroka

§ Information letter № 51-2016 "Submicroscopic changes of the liver under the conditions of induced oncogenesis on the background of cytostatics" N. Lisnychuk, K. Volkov, I. Demkiv, Yu. Soroka

§ Information letter № 278-2017 "Biological markers of endotoxemia in the conditions of induced oncogenesis" N. Lisnychuk, I. Andriychuk, Yu. Soroka

§ Information letter № 301–2017 "Changes in the morphological structure of the spleen in the dynamics of neoplastic lesions" Yu. Soroka, N. Lisnychuk, K. Volkov, Z. Nebesna, I. Andriychuk